Guinea pig insulin (INS) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL011742GU

Alternative Names

insulin, ILPR, IRDN, OTTHUMP00000011162, OTTHUMP00000196038, proinsulin

Reactivity

Guinea pig

Detection Range

12 uIU/mL-210 uIU/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse TH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-110
EDTA plasma (n=4)	89	84-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	97
	Range %	92-103
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Cavia porcellus

Sensitivity

7.5 uIU/mL

Limit Of Detection

450 nm

UniProt ID	P01329
Antigen	Insulin

Applications

ELISA

Research Area

INS

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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