Matrices listed below were spiked with certain level of IgG2alpha and the recovery rates were calculated by comparing the measured value to the expected amount of IgG2alpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
94
EDTA plasma(n=5)
85-105
95
heparin plasma(n=5)
87-94
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG2alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IgG2alpha ELISA Kit allows for the in vitro quantitative determination of IgG2alpha concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Guinea pig IgG2alpha(Immunoglobulin G2alpha) ELISA Kit