Guinea pig estradiol (E2) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027953GU

Sensitivity

25 pg/mL

Detection Range

20 pg/mL-1600 pg/mL

Reactivity

Guinea pig

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

estradiol (E2)

Recovery

The recovery of rat Fbg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	93-104
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Fbg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	91-103
1:200	Average %	90
	Range %	82-94
1:400	Average %	102
	Range %	91-106
1:800	Average %	101
	Range %	91-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Cavia porcellus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	E2

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10500820	CSB-EQ027953GU	96T	$495	Add to Cart
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