Matrices listed below were spiked with certain level of ADP/Acrp30 and the recovery rates were calculated by comparing the measured value to the expected amount of ADP/Acrp30 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
97
EDTA plasma(n=5)
90-104
100
heparin plasma(n=5)
91-102
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ADP/Acrp30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-101%
85-98%
90-101%
EDTA plasma(n=5)
91-99%
87-101%
89-101%
heparin plasma(n=5)
94-99%
81-100%
83-98%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
ADP/Acrp30 ELISA Kit allows for the in vitro quantitative determination of ADP/Acrp30 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Guinea pig ADP/Acrp30 (Adiponectin) ELISA Kit