GSH (Reduced Glutathione) colorimetric Assay Kit

Mfr.No.

EU2640

Alternative Names

GSH ELISA Kit, Reduced Glutathione ELISA Kit

Reactivity

Universal

Detection Method

Colorimetric

Detection Range

1.56-100umol/L

Sample Type

Cell lysates, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Colorimetric Assay

Assay Duration

20 minutes

Recovery

Add a certain amount of GSH into the sample. Calculate the recovery by comparing the measured value with the expected amount of GSH in the sample.

Sample Type	Recovery Range(%)	Average(%)
serum(n=10)	88-100	95
EDTA plasma(n=10)	88-103	97
Heparin plasma(n=10)	86-102	95

Linearity

Dilute the sample with a certain amount of GSH at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type	1:2	1:4	1:8
serum(n=10)	91-103%	90-101%	85-99%
EDTA plasma(n=10)	95-104%	87-99%	83-95%
Heparin plasma(n=10)	99-105%	89-101%	84-99%

Sensitivity

1.2umol/L

Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(umol/L)	OD-1	OD-2	Average
0	0.464	0.462	0.463
1.562	0.272	0.275	0.274
3.125	0.172	0.174	0.173
6.25	0.125	0.126	0.126
12.5	0.103	0.104	0.104
25	0.092	0.092	0.092
50	0.083	0.083	0.083
100	0.079	0.08	0.08

Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5)	37°C for 1 month	2-8°C for 6 months
Average(%)	80	95-100

Notes

Specifically binds with GSH , no obvious cross reaction with other analogues.

UniProt ID	CAS: 70-18-8
Usage Notes	Microplate reader (wavelength: 450nm) 37°C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10uL, 5-50uL, 20-200uL, 200-1000uL) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (umol/L)	3	13.36	50.16	3.04	13.9	51.47
Standard deviation	0.13	0.57	2.35	0.16	0.77	3.25
CV(%)	4.23	4.23	4.68	5.22	5.54	6.32

Storage	2-8°C(Sealed), Don't cryopreserve.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10809817	EU2640	48T	$280		Add to Cart
BHE10809817	EU2640	96T	$400		Add to Cart

GSH (Reduced Glutathione) colorimetric Assay Kit

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