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Home / ELISA Kit

Goat Serum amyloid A(SAA) ELISA Kit

SKU : BHE10500783

This Mouse APOH ELISA Kit was designed for the quantitative measurement of Mouse APOH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E13212G

Reactivity

Goat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Serum amyloid A(SAA)

Recovery

The recovery of mouse Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		92		87-95
EDTA plasma (n=4)		103		98-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1000		Average %		88
		Range %		83-92
1:2000		Average %		97
		Range %		91-101
1:4000		Average %		89
		Range %		84-92
1:8000		Average %		97
		Range %		91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.669	2.625	2.647	2.512
500	2.180	2.112	2.146	2.011
250	1.611	1.600	1.606	1.471
125	0.859	0.831	0.845	0.710
62.5	0.515	0.527	0.521	0.386
31.2	0.321	0.333	0.327	0.192
15.6	0.248	0.242	0.245	0.110
0	0.136	0.134	0.135

Species

Capra hircus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

UniProt ID	A5JST2

Applications	ELISA
Research Area	SAA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Acute-phase proteins in pregnant goats: A longitudinal study Micha Czopowicz.et al,J Vet Diagn Invest.,Jun 23

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SKU	Supplier No.	Size	Your price	Quantity
BHE10500783	CSB-E13212G	96T	$835		Add to Cart
BHE10500783	CSB-E13212G	5x96T	$4008		Add to Cart
BHE10500783	CSB-E13212G	10x96T	$7932.50		Add to Cart

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