Goat Interleukin 4 - IL-4 ELISA Kit

Mfr.No.

E0046GO

Description

This sandwich kit is for the accurate quantitative detection of Goat Interleukin 4 (also known as IL-4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

B-cell stimulatory factor 1;BSF-1;IL 4;IL4;IL-4;Interleukin-4;Lymphocyte stimulatory factor 1

Reactivity

Goat

Detection Range

2-600ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

15137

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.92ng/L

Background

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

Gene ID	100860814
UniProt ID	P79155
Antigen	IL4

Research Area

Immunology; Cancer

Protocol

These standard curves of E0046GO are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	2.199	2.259	2.217
	2.319
160ng/L	1.183	1.189	1.147
	1.195
80ng/L	0.616	0.659	0.617
	0.701
40ng/L	0.399	0.415	0.373
	0.43
20ng/L	0.159	0.165	0.123
	0.171
0ng/L	0.052	0.042	0
	0.032

Precision

We measured random samples of E0046GO within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	183.8	5.69	3.1
Intra-Assay	2	18	127.9	6.5	5.1
Intra-Assay	3	18	73.8	4.54	6.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101370	E0046GO	96T	$458		Add to Cart
BHE12101370	E0046GO	48T	$320		Add to Cart

Goat Interleukin 4 - IL-4 ELISA Kit

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