Goat insulin-like growth factor 1(IGF-1)ELISA Kit

Mfr.No.

CSB-E15909G

Sensitivity

20 ng/mL

Detection Range

80 ng/mL-1000 ng/mL

Reactivity

Goat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

insulin-like growth factor 1 (somatomedin C), IGF1A, IGFI, OTTHUMP00000195084, insulin-like growth factor 1

Recovery

The recovery of mouse TNF-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNF-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	95
	Range %	91-105
1:4	Average %	100
	Range %	92-110
1:8	Average %	92
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.793	2.822	2.808	2.651
500	1.889	1.943	1.916	1.759
250	1.245	1.211	1.228	1.071
125	0.729	0.686	0.708	0.551
62.5	0.373	0.404	0.388	0.232
31.2	0.287	0.298	0.292	0.136
15.6	0.231	0.244	0.238	0.081
0	0.152	0.161	0.157	?

Species

Capra hircus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P51457
Tissue Specificity	Expressed in all tissues examined: brain, lung, liver, spleen, uterus, ovary, testis, heart and skeletal muscle.

Applications	ELISA
Research Area	IGF1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The reproductive performance of female goats treated with melatonin is not improved after introduction to bucks displaying springtime sexual activity if these does are experiencingDecreasing body weight/condition score Zarazaga LA.et al,Anim Reprod Sci.,Feb 6 Intraarticular injection autologous platelet-rich plasma and boneMarrow concentrate in a goat osteoarthritis model Wang Z.et al,J Orthop Res,Feb 21

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