Goat Immunoglobulin G,IgG ELISA Kit

Goat Immunoglobulin G,IgG ELISA Kit

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Mfr.No.

CSB-E12734G

Alternative Names

Immunoglobulin G,IgG

Reactivity

Goat

Detection Range

0.146 ug/mL-37.5 ug/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of pig C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	96	92-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	99
	Range %	91-109
1:2	Average %	87
	Range %	82-93
1:4	Average %	102
	Range %	98-106
1:8	Average %	98
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
600	2.385	2.284	2.335	2.160
300	1.903	1.802	1.853	1.678
150	1.376	1.355	1.366	1.191
75	0.903	0.914	0.909	0.734
37.5	0.650	0.661	0.656	0.481
18.75	0.411	0.422	0.417	0.242
9.375	0.317	0.312	0.315	0.140
0	0.175	0.174	0.175	?

Species

Capra hircus

Sensitivity

0.134 ug/mL

Limit Of Detection

450 nm

Antigen	Immunoglobulin G,IgG

Applications

ELISA

Research Area

IgG

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Intrinsic Inflammation Is a Potential Anti-Epileptogenic Target in the Organotypic Hippocampal Slice Model Chong SA.et al,Neurotherapeutics,Feb 20

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SKU	Supplier No.	Size	Your price
BHE10500753	CSB-E12734G	96T	$395	Add to Cart
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BHE10500753	CSB-E12734G	10x96T	$3752.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.