Matrices listed below were spiked with certain level of GnRH and the recovery rates were calculated by comparing the measured value to the expected amount of GnRH in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
93
EDTA plasma(n=5)
86-103
96
heparin plasma(n=5)
88-98
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GnRH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-102%
85-105%
85-105%
EDTA plasma(n=5)
85-99%
83-94%
88-100%
heparin plasma(n=5)
84-98%
80-97%
82-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
GnRH ELISA Kit allows for the in vitro quantitative determination of GnRH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Goat GnRH (Gonadotropin Releasing Hormone) ELISA Kit