Goat follicle-stimulating hormone,FSH ELISA Kit

Goat follicle-stimulating hormone,FSH ELISA Kit

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Mfr.No.

CSB-E13277G

Alternative Names

follicle stimulating hormone, beta polypeptide, follicle-stimulating hormone beta subunit, follitropin, beta chain, follicle stimulating hormone, beta polypeptide, follicle-stimulating hormone beta subunit, follitropin, beta chain

Reactivity

Goat

Detection Range

20 mIU/mL-800 mIU/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human ENHO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	80-94
EDTA plasma (n=4)	100	94-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ENHO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	94
	Range %	88-99
1:200	Average %	90
	Range %	84-95
1:400	Average %	98
	Range %	91-99
1:800	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.074	2.099	2.087	1.923
50	1.798	1.765	1.782	1.618
25	1.397	1.384	1.391	1.227
12.5	0.982	0.943	0.963	0.799
6.25	0.583	0.596	0.590	0.426
3.12	0.371	0.391	0.381	0.217
1.56	0.296	0.281	0.289	0.125
0	0.162	0.166	0.164	?

Species

Capra hircus

Sensitivity

20 mIU/mL

Limit Of Detection

450 nm

UniProt ID	Q8HY83
Antigen	follicle stimulating hormone, beta polypeptide

Applications

ELISA

Research Area

FSHB

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500739	CSB-E13277G	96T	$790	Add to Cart
BHE10500739	CSB-E13277G	5x96T	$3792	Add to Cart
BHE10500739	CSB-E13277G	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.