Goat adiponectin(ADP)ELISA Kit

Mfr.No.

CSB-E15981G

Alternative Names

adiponectin(ADP)

Reactivity

Goat

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of rat VIM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	95-107
EDTA plasma (n=4)	90	85-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat VIM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-95
1:2	Average %	93
	Range %	88-98
1:4	Average %	97
	Range %	93-102
1:8	Average %	100
	Range %	95-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	3.066	2.888	2.977	2.880
5	2.598	2.621	2.610	2.513
2.5	1.958	1.845	1.902	1.805
1.25	0.943	0.978	0.961	0.864
0.625	0.623	0.612	0.618	0.521
0.312	0.366	0.356	0.361	0.264
0.156	0.228	0.229	0.229	0.132
0	0.093	0.101	0.097	?

Species

Capra hircus

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	U6A087
Antigen	adiponectin(ADP)

Applications

ELISA

Research Area

ADP

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of heat stress on adipokines and some blood metabolites in goats from Jordan. Al-Dawood A.et al,Anim Sci J.,/

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SKU	Supplier No.	Size	Your price
BHE10500715	CSB-E15981G	96T	$595	Add to Cart
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BHE10500715	CSB-E15981G	10x96T	$5652.50	Add to Cart

Goat adiponectin(ADP)ELISA Kit

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