Matrices listed below were spiked with certain level of GLP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of GLP-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
98
EDTA plasma(n=5)
88-105
99
heparin plasma(n=5)
86-100
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GLP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-105%
85-93%
88-105%
EDTA plasma(n=5)
83-97%
89-101%
82-97%
heparin plasma(n=5)
83-98%
80-89%
84-94%
Sensitivity
< 18.75pmol/L
Notes
For Research Use Only
UniProt ID
P01275
Applications
ELISA
Applications Notes
GLP-1ELISA Kit allows for the in vitro quantitative determination of GLP-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of GLP-1(Glucagon Like Peptide 1)ELISA Kit