Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Competitive
Recovery
Matrices listed below were spiked with certain level of GAGs and the recovery rates were calculated by comparing the measured value to the expected amount of GAGs in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-105
97
EDTA plasma(n=5)
89-100
95
heparin plasma(n=5)
86-97
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAGs and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-103%
85-99%
85-96%
EDTA plasma(n=5)
84-101%
82-92%
85-98%
heparin plasma(n=5)
84-99%
81-99%
81-100%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
GAGsELISA Kit allows for the in vitro quantitative determination of GAGs concentrations in serum, plasma, tissue homogenates and other biological fluids.