Fish Tumor Necrosis Factor alpha(TNF-alpha) ELISA KIT

Fish Tumor Necrosis Factor alpha(TNF-alpha) ELISA KIT

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Mfr.No.

CSB-E13254Fh

Sensitivity

125 pg/mL

Detection Range

125 pg/mL-5000 pg/mL

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Tumor Necrosis Factor alpha(TNF-alpha), DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, cachectin, tumor necrosis factor alpha

Recovery

The recovery of rat Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	83-112
EDTA plasma (n=4)	95	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	80-105
1:2	Average %	96
	Range %	85-104
1:4	Average %	102
	Range %	92-114
1:8	Average %	99
	Range %	80-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	3.121	3.129	3.125	2.986
600	2.532	2.522	2.527	2.388
300	1.739	1.765	1.752	1.613
150	1.050	1.041	1.046	0.907
75	0.623	0.642	0.633	0.494
37.5	0.435	0.447	0.441	0.302
18.75	0.265	0.254	0.260	0.121
0	0.135	0.143	0.139	?

Species

Fish

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8JFG3

Applications	ELISA
Research Area	TNF-alpha

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,466-72 Cytokine Responses in Gills of Capoeta umbla as Biomarkers of Environmental Pollution Danabas D.et al,Water Environ Res.,2016 88(3):217-22. Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,May 9

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SKU	Supplier No.	Size	Your price
BHE10500713	CSB-E13254Fh	96T	$835	Add to Cart
BHE10500713	CSB-E13254Fh	5x96T	$4008	Add to Cart
BHE10500713	CSB-E13254Fh	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.