Fish Tri-iodothyronine,T3 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08488f

Alternative Names

Tri-iodothyronine,T3

Detection Range

0.5 ng/mL-8 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human ucMGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	90	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ucMGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	85-98
1:2000	Average %	97
	Range %	91-105
1:4000	Average %	92
	Range %	85-99
1:8000	Average %	93
	Range %	86-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Fish

Sensitivity

0.5 ng/mL

Limit Of Detection

450 nm

Antigen	Tri-iodothyronine,T3

Applications

ELISA

Research Area

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Mild hypothyroidism improves glucose tolerance in experimental type 2 diabetes Ashwini S et al,Chem Biol Interact,2015 Jun 25;235:47-55. Effects of melatonin and green-wavelength LED light on the physiological stress and immunity of goldfish, Carassius auratus, exposed to high water temperature Jung SJ.et al,Fish Physiol Biochem.,2016 Mar 24 Changes of physiological rhythms of N-methyl-d-aspartate receptors in the chum salmon Oncorhynchus keta: effect of seawater acclimation during the parr-smolt transformation Young Jae Choi.et al,Biological Rhythm Research,2016 47(1):77-91.

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