Fish Testosterone(T) ELISA Kit

The recovery of human ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)

Mfr.No.

CSB-E17554Fh

Alternative Names

Testosterone(T)

Detection Range

0.1 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-97
1:2	Average %	98
	Range %	94-103
1:4	Average %	97
	Range %	90-105
1:8	Average %	87
	Range %	82-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Fish

Sensitivity

0.05 ng/mL

Limit Of Detection

450 nm

Antigen	Testosterone(T)

Applications

ELISA

Research Area

Testosterone

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Anti-diabetic effects of shubat in type 2 diabetic rats induced by combination of High-glucose-fat diet and low-dose streptozotocin Manaer T et al,J Ethnopharmacol,2015 Jul 1;169:269-74 2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus) Chen R.et al,Environmental Pollution,/ Ctrp9 and adiponectin receptors in Nile tilapia (Oreochromis niloticus): Molecular cloning, tissue distribution and effects on reproductive genes Guokun Yang.et al,General and Comparative Endocrinology,June 1

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