Fish metallothionein(MT)ELISA kit

Mfr.No.

CSB-EQ027262FI

Sensitivity

12.5 pg/mL

Detection Range

25 pg/mL-400 pg/mL

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

metallothionein(MT)

Recovery

The recovery of human CT-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-99
EDTA plasma (n=4)	95	87-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CT-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	87-105
1:2	Average %	95
	Range %	90-102
1:4	Average %	89
	Range %	83-97
1:8	Average %	95
	Range %	85-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Fish

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	MT

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10500702	CSB-EQ027262FI	96T	$790	Add to Cart
BHE10500702	CSB-EQ027262FI	5x96T	$3792	Add to Cart
BHE10500702	CSB-EQ027262FI	10x96T	$7505	Add to Cart