Fish Interleukin 1beta (IL- 1beta) ELISA Kit

Mfr.No.

CSB-E13259Fh

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Interleukin 1beta (IL- 1beta), Interleukin 1beta, IL-1beta

Recovery

The recovery of human C1S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	110	98-114
EDTA plasma (n=4)	98	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C1S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	100
	Range %	91-104
1:200	Average %	107
	Range %	98-110
1:400	Average %	88
	Range %	82-93
1:800	Average %	96
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Fish

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9YGD3

Applications	ELISA
Research Area	IL1B

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,466-72 Cytokine Responses in Gills of Capoeta umbla as Biomarkers of Environmental Pollution Danabas D.et al,Water Environ Res.,2016 88(3):217-22. Administration of a Polyphenol-Enriched Feed to Farmed Sea Bass (Dicentrarchus labrax L.) Modulates Intestinal and Spleen Immune Responses. Magrone T.et al,Oxid Med Cell Longev.,2016 :2827567. Functional and Structural Characterization of Zebrafish ASC Yajuan Li.et al,FEBS J,May 23

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