Fish immunoglobulin M (IgM) ELISA Kit

Fish immunoglobulin M (IgM) ELISA Kit

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Mfr.No.

CSB-E12045Fh

Sensitivity

1.25 ug/mL

Detection Range

1.25 ug/mL-50 ug/mL

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

immunoglobulin M

Recovery

The recovery of pig ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-99
EDTA plasma (n=4)	93	91-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-94
1:2	Average %	96
	Range %	92-100
1:4	Average %	83
	Range %	80-86
1:8	Average %	91
	Range %	83-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
30	0.153	0.165	0.159
15	0.248	0.240	0.244
7.5	0.534	0.511	0.523
3.75	1.133	1.135	1.134
1.875	2.340	2.260	2.300

Species

Fish

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IgM

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Dietary threonine requirement of juvenile Nile tilapia, Oreochromis niloticus Liebert F et al,J Anim Physiol Anim Nutr (Berl),2009 Dec 93(6):787-93. Probiotic applications of two dominant gut Bacillus strains with antagonistic activity improved the growth performance and immune responses of grouper Epinephelus coioides /,fish & shellfish immunology,2010 Nov;29(5):803-9. The use of kefir as potential probiotic in Çoruh trout (Salmo coruhensis): Effects on growth performance and immunoglobulin (IgM) levels Erkan Can et al,African Joual of Biotechnology,2012 11(30):7775-7780 Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita Giri SS et al,Fish Shellfish Immunol,2013 Feb;34(2):660-6. Effects of dietary supplementation of Lactobacillus pentosus PL11 on the growth performance, immune and antioxidant systems of Japanese eel Anguilla japonica challenged with Edwardsiella tarda Lee JS et al,Fish Shellfish Immunol,2013 Mar;34(3):756-61. Effects of dietary supplementation of potential probiotic Pseudomonas aeruginosa VSG-2 on the innate immunity and disease resistance of tropical freshwater fish, Labeo rohita Lee JS et al,Fish Shellfish Immunol,2012 Jun;32(6):1135-40. Dietary leucine requirement of Juvenile Nile tilapia, Oreochromis niloticus Sarker PK. et al,Fish Physiol Biochem,Aug;38(4):1183-93. Histopathological, Immunological, Hematological and Biochemical Effects of Fipronil on Nile Tilapia (Oreochromis Niloticus) Abd elhakeem El-Murr.et al,Joual of Veterinary Science & Technology,2015 ,Aug Early steps in the European eel (Anguilla anguilla)-Vibrio vulnificus interaction in the gills: role of the RtxA13 toxin. Callol A.et al,Fish Shellfish Immunol.,2015 43(2):502-9. Effects of dietary protein levels on growth performance and haemato-immunological parameters of juvenile genetically improved farmed tilapia (GIFT), Oreochromis niloticus Mathew Didlyn Kpundeh et al,Aquaculture Inteational,2015 23(5): 1189-1201 Effects of dietary Persian oak (Quercus brantii var. persica) fruit extract on survival, growth performance, haematological and immunological parameters in rainbow trout, Oncorhynchus mykiss, fingerlings S. Bohlouli et al,Aquaculture Nutrition,/ Enhanced NK cell adoptive antitumor effects against breast cancer in vitro via blockade of the transforming growth factor-ß signaling pathway Zhao Y. et al,Onco Targets Ther,2015 Jun 22;8:1553-9. Influence of diets supplemented with pomegranate peel extract on performance in Oreochromus niloticus Badawi M E.et al,Japanese Joual of Veterinary Research,64(Supplement 2): S87-S94. Enhancement of Antioxidant Activity, Non-specific Immunity and GrowthPerformance of Nile Tilapia, Oreochromis Niloticus by DietaryFructooligosaccharide Eman A Abd El-Gawad1.et al,J Aquac Res Development,,7(427): 2. Effects of melatonin and green-wavelength LED light on the physiological stress and immunity of goldfish, Carassius auratus, exposed to high water temperature Jung SJ.et al,Fish Physiol Biochem.,2016 Mar 24 Effect of Oyster Mushroom, Pleurotus ostreatus, Extract on Hemato-Immunological Parameters of Rainbow Trout, Oncorhynchus mykiss Uluky G.et al,Joual of the World Aquaculture Society,/ The Effect of Kefir as a Dietary Supplement on Nonspecific Immune Response and Disease Resistance in Juvenile Rainbow Trout, Oncorhynchus mykiss (Walbaum 1792) Uluky G.et al,Joual of the World Aquaculture Society,/ Probiotic efficiency of Bacillus sp. in Labeo rohita challenged by Aeromonas hydrophila: assessment of stress profile, haemato-biochemical parameters and immune responses Ankita Nandi.et al,aquaculture research ,Mar 10 Effects of potential probiotic Bacillus subtilisKADR1 and its subcellular components on immune responses and disease resistance in Labeo rohita Dharmaraj Ramesh.et al,Aquaculture Research,Aug 30 Supplementation of heat-inactivated Bacillus clausii DE5 in diets for grouper, Epinephelus coioides, improves feed utilization, intestinal and systemic immune responses and not growth performance J. Wang.et al,Aquaculture Nutrition,Sep 4 Evaluation of In Vivo Probiotic Efficiency of Bacillus amyloliquefaciens in Labeo rohita Challenged by Pathogenic Strain of Aeromonas hydrophila MTCC 1739 Nandi A.et al,Probiotics Antimicrob Proteins,Jul 25 Comparative study of three predominant gut Bacillus strains and a commercial B. amyloliquefaciens as probiotics on the performance of Clarias gariepinus Rasha M.Reda.et al,Fish & Shellfish Immunology,June 18 Effect of dietary yeast nucleotide on antioxidant activity, non-specific immunity, intestinal cytokines, and disease resistance in Nile Tilapia Rasha M.et al,Fish & Shellfish Immunology,June 9 Effects of a commercial probiotic BS containing Bacillus subtilis and Bacillus licheniformis on growth, immune response and disease resistance in Nile tilapia, Oreochromis niloticus Emmanuel Delwin Abarike .et al,Fish & Shellfish Immunology,August 17 Effects of dietary yeast culture on growth performance, immune response and disease resistance of gibel carp (Carassius auratus gibelio CAS Ⅲ) Peiyu Zhang .et al,Fish & Shellfish Immunology,August 23 Dietary propionic acid enhances antibacterial and immunomodulatory effects of oxytetracycline on Nile tilapia, Oreochromis niloticus El-Adawy M.et al,Environmental Science and Pollution Research,Octobert 4 EVALUATION OF SPIRULINA PLATENSIS ENRICHED DIET ON GROWTH PERFORMANCE, BIOCHEMICAL PARAMETERS AND IMMUNE RESPONSE OF OREOCHROMIS NILOTICUS Abd-El Alim F Abd-El Alim, et al,Slovenian Veterinary Research,September Effect of Dietary Tryptophan Levels on Growth and Hemato-Immunological Parameters in Juvenile Nile Tilapia Oreochromis niloticus Wu Y, et al,The Israeli Joual of Aquaculture - Bamidgeh,/

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.