Fish Heat Shock Protein 70(HSP-70)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E16327Fh

Alternative Names

Heat Shock Protein 70(HSP-70)

Detection Range

18.75 pg/mL-300 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human CYP7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	91	85-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CYP7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	94
	Range %	90-100
1:4	Average %	92
	Range %	85-97
1:8	Average %	92
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Fish

Sensitivity

18.75 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9I8F9
Antigen	Heat Shock Protein 70(HSP-70)

Applications

ELISA

Research Area

HSP70

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Vessel noise pollution as a human threat to fish: assessment of the stress response in gilthead sea bream (Sparus aurata, Linnaeus 1758) Celi M. et al,Fish Physiol Biochem,2015 Therapeutic effects of C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid (CDDO-Me; bardoxolone methyl) on radiation-induced lung inflammation and fibrosis in mice Wang YY. et al,Drug Des Devel Ther,2015 Jun 22;9:3163-78. Dietary supplementation of vitamin C: an effective measure to give protection against UV-B irradiation using fish as model organism Rina Chakrabarti, et al,Photochemical & Photobiological Sciences,November 5 INVESTIGATING THE ROLE OF HEAT SHOCK PROTEINS AND MELANOPHORES DURING STRESS CAUSED BY HIGH LIGHT INTENSITY IN INDIAN MAJOR CARP, CATLA CATLA N. A. Khan, et al,International Journal of Pharmaceutical Sciences and Research,December 1

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