Fish dopamine(DA) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027496FI

Sensitivity

62.5 pg/mL

Detection Range

62.5 pg/mL-1000 pg/mL

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

dopamine(DA)

Recovery

The recovery of rat C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	95	89-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	81-89
1:200	Average %	92
	Range %	87-96
1:400	Average %	101
	Range %	97-106
1:800	Average %	87
	Range %	84-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Fish

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	DA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Ambient particulate air pollution and circulating antioxidant enzymes: A repeated-measure study in healthy adults in Beijing, China Shaowei Wu. et al,Environ Pollut,2015 Jun 11.pii: S0269-7491(15)00280-8. Dopamine regulates renal osmoregulation during hyposaline stress via DRD1 in the spotted scat (Scatophagus argus) Maoliang Su.et al,Scientific Reports,Nov 18;6:37535 Changes of physiological rhythms of N-methyl-d-aspartate receptors in the chum salmon Oncorhynchus keta: effect of seawater acclimation during the parr-smolt transformation Young Jae Choi.et al,Biological Rhythm Research,2016 47(1):77-91.

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SKU	Supplier No.	Size	Your price
BHE10500683	CSB-EQ027496FI	96T	$790	Add to Cart
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BHE10500683	CSB-EQ027496FI	10x96T	$7505	Add to Cart