Fish Complement 3,C3 ELISA Kit

Mfr.No.

CSB-E09727s

Alternative Names

Complement 3,C3

Detection Range

12.5 ug/mL-500 ug/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human AOAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	101	94-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AOAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-96
1:2	Average %	97
	Range %	91-103
1:4	Average %	99
	Range %	82-106
1:8	Average %	87
	Range %	81-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Fish

Sensitivity

12.5 ug/mL

Limit Of Detection

450 nm

Antigen	Complement 3,C3

Applications

ELISA

Research Area

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10500680	CSB-E09727s	10x96T	$8132	Add to Cart