Fish Aromatase(ARO) ELISA Kit

Fish Aromatase(ARO) ELISA Kit

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Mfr.No.

CSB-EL006394FI

Detection Range

25 pg/mL-1000 pg/mL

Sensitivity

25 pg/mL

Sample Type

Serum, Tissue homogenates, Plasma

Assay Duration

1-5h

Alternative Names

cytochrome P450, family 19, subfamily A, polypeptide 1, ARO, ARO1, CPV1, CYAR, CYP19, MGC104309, P-450AROM, OTTHUMP00000162543, aromatase, cytochrome P450, subfamily XIX (aromatization of androgens), estrogen synthetase, flavoprotein-linked monooxygenase, mic

Recovery

The recovery of human C4BPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-105
EDTA plasma (n=4)	95	89-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C4BPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	91-108
1:2000	Average %	92
	Range %	87-99
1:4000	Average %	94
	Range %	89-98
1:8000	Average %	97
	Range %	91-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.905	2.875	2.890	2.755
100	2.489	2.473	2.481	2.346
50	1.734	1.750	1.742	1.607
25	0.948	0.957	0.953	0.818
12.5	0.512	0.506	0.509	0.374
6.25	0.464	0.471	0.468	0.333
3.125	0.230	0.234	0.232	0.097
0	0.133	0.136	0.135

Species

Fish

Limit Of Detection

450 nm

UniProt ID	Q92087
Antigen	cytochrome P450, family 19, subfamily A, polypeptide 1

Applications

ELISA

Research Area

CYP19A1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	New inflammatoryMarkers for prediction of non-dipper blood pressure pattern in patients with essential hypertension: Serum YKL-40/Chitinase 3-like protein 1 levels and echocardiographic epicardial adipose tissue thickness Eftal Murat Bakirci et al,/,2015 Oct;37(6):505-10.

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SKU	Supplier No.	Size	Your price
BHE10500677	CSB-EL006394FI	96T	$790	Add to Cart
BHE10500677	CSB-EL006394FI	5x96T	$3792	Add to Cart
BHE10500677	CSB-EL006394FI	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.