Fish adrenocorticotropic hormone (ACTH) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E15926Fh

Sensitivity

75 pg/mL

Detection Range

75 pg/mL-1200 pg/mL

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

adrenocorticotropic hormone (ACTH)

Recovery

The recovery of human FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	96	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	97
	Range %	92-104
1:200	Average %	95
	Range %	88-100
1:400	Average %	96
	Range %	89-103
1:800	Average %	93
	Range %	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Fish

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	ACTH

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Vessel noise pollution as a human threat to fish: assessment of the stress response in gilthead sea bream (Sparus aurata, Linnaeus 1758) Celi M. et al,Fish Physiol Biochem,2015 LH-Independent Testosterone Secretion Is Mediated by the Interaction Between GNRH2 and Its Receptor Within Porcine Testes Desaulniers AT. et al,Biol Reprod.,2015 Aug;93(2):45. Profiles of hypothalamus-pituitary-interrenal axis gene expression in the parr and smolt stages of rainbow trout, Oncorhynchus mykiss: Effects of recombinant aquaporin 3 and seawater acclimation Young Jae Choi.et al,Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology,182: 14-21 Regulation of divergent cortisol responsiveness in European sea bass, Dicentrarchus labrax L. Athanasios Samaras .et al,plos one,August 10

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BHE10500673	CSB-E15926Fh	10x96T	$7505	Add to Cart