Matrices listed below were spiked with certain level of F-E3 and the recovery rates were calculated by comparing the measured value to the expected amount of F-E3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-105
96
EDTA plasma(n=5)
94-105
98
heparin plasma(n=5)
88-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-E3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
F-E3 ELISA Kit allows for the in vitro quantitative determination of F-E3 concentrations in serum, plasma, tissue homogenates and other biological fluids.