Human Von Willebrand Factor (vWF) (Competitive EIA) ELISA Kit

Mfr.No.

CEA833Hu

Sensitivity

5.22ng/mL

Detection Range

12.35-1,000ng/mL

Reactivity

Human

Assay Duration

Assay Type

Competitive Inhibition

Description

This assay has high sensitivity and excellent specificity for detection of Von Willebrand Factor (vWF).
No significant cross-reactivity or interference between Von Willebrand Factor (vWF) and analogues was observed.

Alternative Names

F8VWF; VWD; von Willebrand antigen 2

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Reagent Diluent	1×300µL	Stop Solution	1×6mL
TMB Substrate	1×9mL	Instruction manual	1
Wash Buffer (30 × concentrate)	1×20mL

Recovery

Matrices listed below were spiked with certain level of recombinant Von Willebrand Factor (vWF) and the recovery rates were calculated by comparing the measured value to the expected amount of Von Willebrand Factor (vWF) in samples.

Matrix	Recovery range (%)	Average(%)
sodium citrate plasma(n=5)	97-104	101

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Von Willebrand Factor (vWF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
sodium citrate plasma(n=5)	78-98%	93-101%	80-105%	81-99%

Features

Detects small molecule

Species

Human

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Von Willebrand Factor (vWF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Von Willebrand Factor (vWF) and unlabeled Von Willebrand Factor (vWF) (Standards or samples) with the pre-coated antibody specific to Von Willebrand Factor (vWF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Protocol

Datasheet

Gene ID	7450
UniProt ID	P04275

Applications	ELISA
Research Area	Signal transduction

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Reference	Differential integrative omic analysis for mechanism insights and biomarker discovery of abnormal Savda syndrome and its unique Munziq prescription

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SKU	Supplier No.	Size	Your price
BHE10102885	CEA833Hu	96T*1	$630	Add to Cart
BHE10102885	CEA833Hu	96T*5	$2835	Add to Cart
BHE10102885	CEA833Hu	96T*10	$5355	Add to Cart

ELISA Kit for Von Willebrand Factor (vWF) (Human)

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