Human Transforming Growth Factor Beta 3 (TGFb3) (Sandwich ELISA) ELISA Kit

Mfr.No.

SEB949Hu

Description

This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 3 (TGFb3).
No significant cross-reactivity or interference between Transforming Growth Factor Beta 3 (TGFb3) and analogues was observed.

Alternative Names

TGF-B3; LAP; Latency-associated peptide

Reactivity

Human

Detection Method

Colormetric

Detection Range

15.6-1,000pg/mL

Sample Type

Cell lysates, Cell culture supernates, Tissue homogenates, Other biological fluids

Assay Type

Sandwich

Assay Duration

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Transforming Growth Factor Beta 3 (TGFb3) and the recovery rates were calculated by comparing the measured value to the expected amount of Transforming Growth Factor Beta 3 (TGFb3) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	78-95	82
EDTA plasma(n=5)	80-96	90
heparin plasma(n=5)	81-98	85

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transforming Growth Factor Beta 3 (TGFb3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	93-101%	95-102%	99-105%	98-105%
EDTA plasma(n=5)	90-101%	94-102%	81-91%	87-95%
heparin plasma(n=5)	95-105%	83-95%	97-105%	86-101%

Features

Detects protein (regular version)

Species

Human

Sensitivity

5.7pg/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 3 (TGFb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 3 (TGFb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 3 (TGFb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 3 (TGFb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Gene ID	7043
UniProt ID	P10600
Antigen	TGFb3
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Applications	ELISA
Research Area	Cytokine
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 3 (TGFb3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 3 (TGFb3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: C<10% Inter-Assay: C<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Reference	A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches The immunomodulating effect of seminal plasma on T cells Influence of vitamin D and transforming growth factor β3 serum concentrations, obesity, and family history on the risk for uterine fibroids New scaffolds encapsulating TGF-β3/BMP-7 combinations driving strong chondrogenic differentiation

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SKU	Supplier No.	Size	Your price
BHE10104736	SEB949Hu	96T*1	$665	Add to Cart
BHE10104736	SEB949Hu	96T*5	$2992.50	Add to Cart
BHE10104736	SEB949Hu	96T*10	$5652.50	Add to Cart

ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) (Human)

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