Mouse Phospholipase A2 Group VII (LpPLA2) (Sandwich ELISA) ELISA Kit

Mfr.No.

SEA867Mu

Description

This assay has high sensitivity and excellent specificity for detection of Phospholipase A2 Group VII (LpPLA2).
No significant cross-reactivity or interference between Phospholipase A2 Group VII (LpPLA2) and analogues was observed.

Alternative Names

PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2; Phospholipase A2, Lipoprotein Associated

Reactivity

Mouse

Detection Method

Colormetric

Detection Range

0.312-20ng/mL

Sample Type

Other biological fluids, Tissue homogenates, Cell lysates, Cell culture supernates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Phospholipase A2 Group VII (LpPLA2) and the recovery rates were calculated by comparing the measured value to the expected amount of Phospholipase A2 Group VII (LpPLA2) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	92-102	98
EDTA plasma(n=5)	94-105	99
heparin plasma(n=5)	83-91	87

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Phospholipase A2 Group VII (LpPLA2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	89-97%	89-96%	98-105%	87-103%
EDTA plasma(n=5)	84-99%	93-102%	87-97%	80-93%
heparin plasma(n=5)	78-97%	79-101%	88-96%	83-92%

Features

Detects protein (regular version)

Species

Mouse

Sensitivity

0.114ng/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2 Group VII (LpPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2 Group VII (LpPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2 Group VII (LpPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2 Group VII (LpPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Gene ID	27226
UniProt ID	Q60963
Antigen	LpPLA2
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Applications	ELISA
Research Area	Enzyme///Kinase
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2 Group VII (LpPLA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2 Group VII (LpPLA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10103007	SEA867Mu	96T*1	$648	Add to Cart
BHE10103007	SEA867Mu	96T*5	$2916	Add to Cart
BHE10103007	SEA867Mu	96T*10	$5508	Add to Cart

ELISA Kit for Phospholipase A2 Group VII (LpPLA2) (Mouse)

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