Human Cross Linked C-Telopeptide Of Type II Collagen (CTXII) (Competitive EIA) ELISA Kit

Mfr.No.

CEA686Hu

Sensitivity

52.3pg/mL

Detection Range

123.5-10,000pg/mL

Reactivity

Human

Assay Duration

Assay Type

Competitive Inhibition

Description

This assay has high sensitivity and excellent specificity for detection of Cross Linked C-Telopeptide Of Type II Collagen (CTXII).
No significant cross-reactivity or interference between Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and analogues was observed.

Alternative Names

CTX-II; CTX2

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Reagent Diluent	1×300µL	Stop Solution	1×6mL
TMB Substrate	1×9mL	Instruction manual	1
Wash Buffer (30 × concentrate)	1×20mL

Recovery

Matrices listed below were spiked with certain level of recombinant Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and the recovery rates were calculated by comparing the measured value to the expected amount of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	78-94	82
EDTA plasma(n=5)	98-105	101
heparin plasma(n=5)	89-96	92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	89-98%	89-105%	78-94%	93-101%
EDTA plasma(n=5)	93-101%	79-99%	82-98%	78-97%
heparin plasma(n=5)	79-90%	94-101%	95-102%	78-105%

Features

Detects small molecule

Species

Human

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type II Collagen (CTXII) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type II Collagen (CTXII) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cross Linked C-Telopeptide Of Type II Collagen (CTXII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and unlabeled Cross Linked C-Telopeptide Of Type II Collagen (CTXII) (Standards or samples) with the pre-coated antibody specific to Cross Linked C-Telopeptide Of Type II Collagen (CTXII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Protocol

Datasheet

Gene ID	1280
UniProt ID	P02458

Applications	ELISA
Research Area	Metabolic pathway

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Reference	Comparison of Intra-articular Injection of Hyaluronic Acid and N-Acetyl Cysteine in the Treatment of Knee Osteoarthritis A Pilot Study

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ELISA Kit for Cross Linked C-Telopeptide Of Type II Collagen (CTXII) (Human)

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