Human Anti-Beta-2-Microglobulin Antibody (Anti-b2M) (Competitive EIA) ELISA Kit

Mfr.No.

AEA260Hu

Sensitivity

1.19ng/mL

Detection Range

3.12-200ng/mL

Reactivity

Human

Assay Duration

2h, 30min

Assay Type

Competitive Inhibition

Description

This assay has high sensitivity and excellent specificity for detection of Anti-Beta-2-Microglobulin Antibody (Anti-b2M).
No significant cross-reactivity or interference between Anti-Beta-2-Microglobulin Antibody (Anti-b2M) and analogues was observed.

Alternative Names

BMG; B2MG

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of Anti-Beta-2-Microglobulin Antibody (Anti-b2M) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Beta-2-Microglobulin Antibody (Anti-b2M) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	83-99	93
EDTA plasma(n=5)	85-102	96
heparin plasma(n=5)	85-98	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Beta-2-Microglobulin Antibody (Anti-b2M) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	89-98%	96-105%	78-93%	97-105%
EDTA plasma(n=5)	88-104%	87-101%	93-102%	95-105%
heparin plasma(n=5)	88-97%	86-101%	92-99%	89-96%

Features

Detects protein (antigen)

Species

Human

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Beta-2-Microglobulin Antibody (Anti-b2M) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Beta-2-Microglobulin Antibody (Anti-b2M) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Beta-2-Microglobulin Antibody (Anti-b2M) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Beta-2-Microglobulin Antibody (Anti-b2M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

Gene ID	567
UniProt ID	P61769

Applications	ELISA
Research Area	Infection immunity

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10109625	AEA260Hu	96T*1	$900	Add to Cart
BHE10109625	AEA260Hu	96T*5	$4050	Add to Cart
BHE10109625	AEA260Hu	96T*10	$7650	Add to Cart

ELISA Kit for Anti-Beta-2-Microglobulin Antibody (Anti-b2M) (Human)

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