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Home / ELISA Kit

EliKine Mouse TGF-beta1 ELISA Kit

SKU : BHE13710189

TGF-beta is capable of producing a variety of effects and virtually all cell types respond to this factor in some way. The inappropriate presence of active TGF-beta1 has been implicated in a variety of pathological conditions Because of the necessity for regulating its activity tightly, TGF-beta is secreted by cells in the form of an inactive complex. This complex consists of TGF-beta1 associated non-covalently with a protein designated the latency associated peptide (LAP). TGF-beta1 and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-beta1 each consist of a disulfide-linked homodimer and the association of these two components renders TGF-beta1 inactive and inaccessible to anti-TGF-beta antibodies.

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Mfr.No.	KTE7014
Description	EliKine Mouse TGF-beta1 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse TGF-beta1.
Alternative Names	TGFB1; CED; DPD1; LAP; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide
Reactivity	Mouse, Rat
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Mouse TGF-beta1 microplate Mouse TGF-beta1 standard Mouse TGF-beta1 detect antibody EliKine Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	EliKine Mouse TGF-beta1 ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TGF-beta1. No significant cross-reactivity or interference between Mouse TGF-beta1 and analogues was observed.
Calibration Range	15.6 pg/ml-1000 pg/ml
Limit Of Detection	8 pg/mL
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TGF-beta is capable of producing a variety of effects and virtually all cell types respond to this factor in some way. The inappropriate presence of active TGF-beta1 has been implicated in a variety of pathological conditions Because of the necessity for regulating its activity tightly, TGF-beta is secreted by cells in the form of an inactive complex. This complex consists of TGF-beta1 associated non-covalently with a protein designated the latency associated peptide (LAP). TGF-beta1 and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-beta1 each consist of a disulfide-linked homodimer and the association of these two components renders TGF-beta1 inactive and inaccessible to anti-TGF-beta antibodies.
Notes	EliKine Mouse TGF-beta1 ELISA Kit employs a two-site sandwich ELISA to quantitate TGF-beta1 in samples. An antibody specific for TGF-beta1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-beta1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-beta1 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-beta1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	21803
Tag	TGFB1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	EliKine Mouse TGF-beta1 ELISA Kit employs a two-site sandwich ELISA to quantitate TGF-beta1 in samples. An antibody specific for TGF-beta1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-beta1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-beta1 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-beta1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13710189	KTE7014	48 T	$189		Add to Cart
BHE13710189	KTE7014	96 T	$289		Add to Cart

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