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Home / ELISA Kit

EliKine Mouse IL-22 ELISA Kit

SKU : BHE13710188

IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.

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Mfr.No.	KTE7013
Description	EliKine Mouse IL-22 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-22.
Alternative Names	IL 22; IL22; IL-21; IL-22; IL-D110; IL-TIF; IL21; ILTIF; MGC79382; MGC79384; TIFIL-23; TIFa; zcyto18; IL-10-related T-cell-derived inducible factor; interleukin 21
Reactivity	Mouse
Detection Method	Colorimetric
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Mouse IL-22 microplate Mouse IL-22 standard Mouse IL-22 detect antibody EliKine Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	EliKine Mouse IL-22 ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL-22. No significant cross-reactivity or interference between Mouse IL-22 and analogues was observed.
Calibration Range	15.6 pg/mL-1000 pg/mL
Limit Of Detection	8 pg/mL
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.
Notes	EliKine Mouse IL-22 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-22 in samples. An antibody specific for IL-22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-22 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-22 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	50929
Tag	IL22
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	EliKine Mouse IL-22 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-22 in samples. An antibody specific for IL-22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-22 is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-22 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13710188	KTE7013	48 T	$189		Add to Cart
BHE13710188	KTE7013	96 T	$289		Add to Cart

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