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Home / ELISA Kit

EliKine Mouse IL-1alpha ELISA Kit

SKU : BHE13710179

Interleukin-1 (IL-1) is one of the first cytokines ever described. Its initial discovery was as a factor that could induce fever, control lymphocytes, increase the number of bone marrow cells and cause degeneration of bone joints. At this time, IL-1 was known under several other names including endogenous pyrogen, lymphocyte activating factor, haemopoetin-1 and mononuclear cell factor, amongst others. It was around 1984-1985 when scientists confirmed that IL-1 was actually composed of two distinct proteins, now called IL-1alpha and IL-1beta.These belong to a family of cytokines known as the interleukin-1 superfamily.Both IL-1alpha and IL-1beta are produced by macrophages, monocytes and dendritic cells.

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Mfr.No.	KTE7004
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	EliKine Mouse IL-1alpha ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-1alpha.
Alternative Names	Hematopoietin 1; IL 1 alpha; Il 1a; IL1; IL1 ALPHA; IL1A; IL1F1; Ilia; Interleukin 1 alpha; Interleukin 1 alpha precursor; Interleukin1 alpha
Detection Method	Colorimetric
Kit Components	Mouse IL-1alpha microplate Mouse IL-1alpha standard Mouse IL-1alpha detect antibody EliKine Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	EliKine Mouse IL-1alpha ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL-1alpha. No significant cross-reactivity or interference between Mouse IL-1alpha and analogues was observed.
Calibration Range	7.8 pg/mL-500 pg/mL
Limit Of Detection	4 pg/mL
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Interleukin-1 (IL-1) is one of the first cytokines ever described. Its initial discovery was as a factor that could induce fever, control lymphocytes, increase the number of bone marrow cells and cause degeneration of bone joints. At this time, IL-1 was known under several other names including endogenous pyrogen, lymphocyte activating factor, haemopoetin-1 and mononuclear cell factor, amongst others. It was around 1984-1985 when scientists confirmed that IL-1 was actually composed of two distinct proteins, now called IL-1alpha and IL-1beta.These belong to a family of cytokines known as the interleukin-1 superfamily.Both IL-1alpha and IL-1beta are produced by macrophages, monocytes and dendritic cells.
Notes	EliKine Mouse IL-1alpha ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1alpha in samples. An antibody specific for IL-1alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1alpha is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	16175

Applications	ELISA
Applications Notes	EliKine Mouse IL-1alpha ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1alpha in samples. An antibody specific for IL-1alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1alpha is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13710179	KTE7004	48 T	$189		Add to Cart
BHE13710179	KTE7004	96 T	$289		Add to Cart

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