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Home / ELISA Kit

EliKine Human TSH ELISA Kit

SKU : BHE13710206

Thyroid-stimulating hormone (also known as thyrotropin, thyrotropic hormone, TSH, or hTSH for human TSH) is a pituitary hormone that stimulates the thyroid gland to produce thyroxine (T4), and then triiodothyronine (T3) which stimulates the metabolism of almost every tissue in the body. It is a glycoprotein hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland, which regulates the endocrine function of the thyroid. In 1916, Bennett M. Allen and Philip E. Smith found that the pituitary contained a thyrotropic substance.

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Mfr.No.	KTE6905
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	EliKine Human TSH ELISA Kit employs a two-site sandwich ELISA to quantitate Human TSH.
Alternative Names	Thyrotropin subunit beta; TSH A; TSH alpha; TSH B; TSH beta; TSHA; TSHB; Thyroid stimulating hormone; Thyroid stimulating hormone alpha chain
Detection Method	Colorimetric
Kit Components	Human TSH microplate Human TSH standard Human TSH detect antibody HRP substrate A HRP substrate B Stop solution Wash buffer Plate covers
Features	EliKine Human TSH ELISA Kit has high sensitivity and excellent specificity for detection of Human TSH. No significant cross-reactivity or interference between Human TSH and analogues was observed.
Calibration Range	0.3 mIU/L-12 mIU/L
Limit Of Detection	0.1 mIU/L
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Thyroid-stimulating hormone (also known as thyrotropin, thyrotropic hormone, TSH, or hTSH for human TSH) is a pituitary hormone that stimulates the thyroid gland to produce thyroxine (T4), and then triiodothyronine (T3) which stimulates the metabolism of almost every tissue in the body. It is a glycoprotein hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland, which regulates the endocrine function of the thyroid. In 1916, Bennett M. Allen and Philip E. Smith found that the pituitary contained a thyrotropic substance.
Notes	EliKine Human TSH ELISA Kit employs a two-site sandwich ELISA to quantitate TSH in samples. An antibody specific for TSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for TSH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for

Gene ID	1081

Applications	ELISA
Applications Notes	EliKine Human TSH ELISA Kit employs a two-site sandwich ELISA to quantitate TSH in samples. An antibody specific for TSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for TSH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13710206	KTE6905	48 T	$189		Add to Cart
BHE13710206	KTE6905	96 T	$289		Add to Cart

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