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Home / ELISA Kit

EliKine Human Prostate Specific Antigen (PSA) ELISA Kit

SKU : BHE13710218

PSA is a proteolytic enzyme mainly produced by prostate epithelial cells, which is secreted into prostatic fluid or semen under normal circumstances. Serum PSA determination has high accuracy, stability, good repeatability, and is non-invasive, which helps in the early diagnosis of prostate cancer, monitoring treatment response and judgment of prognosis.

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Mfr.No.	KTE6039
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3.5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	EliKine Human Prostate Specific Antigen (PSA) ELISA Kit employs a two-site sandwich ELISA to quantitate Human PSA
Alternative Names	antigen; prostate-specific ELISA Kit; APS ELISA Kit; Gamma seminoprotein ELISA Kit; Gamma-seminoprotein ELISA Kit; hK3 ELISA Kit; Kallikrein 3 ELISA Kit; Kallikrein related peptidase 3 ELISA Kit; Kallikrein-3 ELISA Kit; KLK 3 ELISA Kit; KLK2A1 ELISA Kit; Klk3 ELISA Kit; KLK3_HUMAN ELISA Kit.
Detection Method	Colorimetric
Kit Components	Human PSA Microplate Human PSA Standard (lyophilized) Sample Diluent (5x) Assay Buffer (5x) HRP-conjugated Human PSA Detect Antibody (100x) HRP Substrate(TMB) Stop Solution Wash Buffer (20x ) Plate Covers
Features	EliKine Human Prostate Specific Antigen (PSA) ELISA Kit has high sensitivity and excellent specificity for detection of Human Prostate Specific antigen (PSA). No significant cross-reactivity or interference between Human Prostate Specific antigen (PSA) and analogues was observed.
Calibration Range	0.078 ng/mL- 5 ng/mL
Sample Type	Serum, Cell culture supeatants, Plasma
Limit Of Detection	0.078 ng/mL
Precautions	The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes.
Background	PSA is a proteolytic enzyme mainly produced by prostate epithelial cells, which is secreted into prostatic fluid or semen under normal circumstances. Serum PSA determination has high accuracy, stability, good repeatability, and is non-invasive, which helps in the early diagnosis of prostate cancer, monitoring treatment response and judgment of prognosis.
Notes	EliKine Human Prostate Specific Antigen (PSA) ELISA Kit employs a double antibody sandwich method to quantitate Human PSA in samples. An antibody specific for Human PSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human PSA present is bound by the immobilized antibody. After removing any unbound substances, a HRP-conjugated antibody specific for Human PSA is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB tus blue under the catalysis of HRP, and tus yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human Carcinoembryonic antigen (PSA) concentration is proportional to the OD450 nm value.
Usage Notes	1. If Sample Diluent (5x) and Assay Buffer (5x) appears to tu yellow or a small amount of precipitation, etc., it is caused by the serum contained in the reagent. Please centrifuge to remove the precipitate, which will not affect normal use. 2. Do not mix or substitute reagents with those from other lots or sources. 3. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. 4. To ensure accurate results, proper adhesion of plate covers during incubation steps is necessary. 5. Stop Solution has certain Corrosive. Please take protective measures when operating.

Applications	ELISA
Applications Notes	EliKine Human Prostate Specific Antigen (PSA) ELISA Kit employs a double antibody sandwich method to quantitate Human PSA in samples. An antibody specific for Human PSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human PSA present is bound by the immobilized antibody. After removing any unbound substances, a HRP-conjugated antibody specific for Human PSA is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB tus blue under the catalysis of HRP, and tus yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human Carcinoembryonic antigen (PSA) concentration is proportional to the OD450 nm value.

Storage Instructions	Stored at 4°C for 12 months
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13710218	KTE6039	48 T	$259		Add to Cart
BHE13710218	KTE6039	96 T	$359		Add to Cart

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