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Home / ELISA Kit

EliKine Human beta-NGF ELISA Kit

SKU : BHE13710169

beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of beta-NGF gene's expression is associated with allergic rhinitis.

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Mfr.No.	KTE6028
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	EliKine Human beta-NGF ELISA Kit employs a two-site sandwich ELISA to quantitate Human beta-NGF.
Alternative Names	Beta nerve growth factor; Beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor (beta polypeptide); Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGFB; NID67
Detection Method	Colorimetric
Kit Components	Human beta-NGF microplate Human beta-NGF standard Human beta-NGF detect antibody EliKine Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	EliKine Human beta-NGF ELISA Kit has high sensitivity and excellent specificity for detection of Human beta-NGF. No significant cross-reactivity or interference between Human beta-NGF and analogues was observed.
Calibration Range	31.25 pg/mL- 2000 pg/mL
Limit Of Detection	16 pg/mL
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of beta-NGF gene's expression is associated with allergic rhinitis.
Notes	EliKine Human beta-NGF ELISA Kit employs a two-site sandwich ELISA to quantitate beta-NGF in samples. An antibody specific for beta-NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-NGF is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4803

Applications	ELISA
Applications Notes	EliKine Human beta-NGF ELISA Kit employs a two-site sandwich ELISA to quantitate beta-NGF in samples. An antibody specific for beta-NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-NGF is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13710169	KTE6028	48 T	$189		Add to Cart
BHE13710169	KTE6028	96 T	$289		Add to Cart

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