JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

EliKine Free Thyroxine (fT4) ELISA Kit

SKU : BHE13710211

Over 99% of Triiodothyronine (T4) circulates in blood is bound to carrier proteins; thyroxine-binding globulin (TBG). However, only the free (unbound) portion of T4 is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T4 level changes so that the free T4 concentration remains constant. Thus, measurements of free T4 concentrations correlate more reliably with clinical status than total T4 levels. The increase in total T4 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T4 levels while the free T4 concentration remains basically unchanged.

Continuing the saving: $50 Off All ELISA Kits Offer Extended!

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE0005
Reactivity	Human, Mouse, Rat, Goat, Sheep, Dog, Rabbit, Bovine, Monkey, Chicken, Swine
Assay Duration	Multiple steps standard competitive ELISA assay with a working time of 3-4 hours. It depends on the experience of the operation person.
Assay Type	Competitive (quantitative)
Description	EliKine fT4 ELISA Kit employs a competitive ELISA to quantitate fT4.
Detection Method	Colorimetric
Kit Components	fT4 microplate fT4 standard Biotin conjugated fT4 Avidin-HRP HRP substrate A HRP substrate B Stop solution Wash buffer Plate covers
Features	EliKine fT4 ELISA Kit has high sensitivity and excellent specificity for detection of fT4. No significant cross-reactivity or interference between fT4 and analogues was observed.
Calibration Range	4 pmol/L-64 pmol/L
Limit Of Detection	4.0 pmol/L
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Over 99% of Triiodothyronine (T4) circulates in blood is bound to carrier proteins; thyroxine-binding globulin (TBG). However, only the free (unbound) portion of T4 is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total T4 level changes so that the free T4 concentration remains constant. Thus, measurements of free T4 concentrations correlate more reliably with clinical status than total T4 levels. The increase in total T4 levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T4 levels while the free T4 concentration remains basically unchanged.
Notes	EliKine fT4 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to fT 4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated fT 4. A competitive inhibition reaction is launched between fT4 (Standards or samples) and Biotin-conjugated fT4 with the pre-coated antibody specific for fT 4. The more amount of fT4 in samples, the less antibody bound by Biotin-conjugated fT 4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of fT4 in the sample. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for

Applications	ELISA
Applications Notes	EliKine fT4 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to fT4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated fT4. A competitive inhibition reaction is launched between fT4 (Standards or samples) and Biotin-conjugated fT4 with the pre-coated antibody specific for fT4. The more amount of fT4 in samples, the less antibody bound by Biotin-conjugated fT4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of fT4 in the sample. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13710211	KTE0005	48 T	$189		Add to Cart
BHE13710211	KTE0005	96 T	$289		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.