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Home / ELISA Kit

EliKine Estradiol ELISA Kit

SKU : BHE13710209

Estradiol (E2), also spelled oestradiol, is a steroid, an estrogen, and the primary female sex hormone. It is named for and is important in the regulation of the estrous and menstrual female reproductive cycles. Estradiol is essential for the development and maintenance of female reproductive tissues such as the breasts, uterus, and vagina during puberty, adulthood, and pregnancy, but it also has important effects in many other tissues, including bone, fat, skin, liver, and the brain. While estrogen levels in men are lower compared to those in women, estrogens have essential functions in men, as well. It is found in most vertebrates and crustaceans, insects, fish, and other animal species.

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Mfr.No.	KTE0003
Reactivity	Donkey, Human
Assay Duration	Multiple steps standard competitive ELISA assay with a working time of 3-4 hours. It depends on the experience of the operation person.
Assay Type	Competitive (quantitative)
Description	EliKine Estradiol ELISA Kit employs a competitive ELISA to quantitate Estradiol.
Alternative Names	E2; 17beta-estradiol; Estrogen
Detection Method	Colorimetric
Kit Components	Estradiol microplate Estradiol standard HRP conjugated estradiol Estradiol detect antibody HRP substrate A HRP substrate B Stop solution Wash buffer Plate covers
Features	EliKine Estradiol ELISA Kit has high sensitivity and excellent specificity for detection of estradiol. No significant cross-reactivity or interference between estradiol and analogues was observed.
Calibration Range	40 pg/ml-1500 pg/ml
Limit Of Detection	40 pg/ml
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Estradiol (E2), also spelled oestradiol, is a steroid, an estrogen, and the primary female sex hormone. It is named for and is important in the regulation of the estrous and menstrual female reproductive cycles. Estradiol is essential for the development and maintenance of female reproductive tissues such as the breasts, uterus, and vagina during puberty, adulthood, and pregnancy, but it also has important effects in many other tissues, including bone, fat, skin, liver, and the brain. While estrogen levels in men are lower compared to those in women, estrogens have essential functions in men, as well. It is found in most vertebrates and crustaceans, insects, fish, and other animal species.
Notes	EliKine Estradiol ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for estradiol and Horseradish Peroxidase (HRP) conjugated estradiol. The competitive inhibition reaction is launched between with HRP labeled estradiol and unlabeled estradiol with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of estradiol in the sample. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for

Applications	ELISA
Applications Notes	EliKine Estradiol ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for estradiol and Horseradish Peroxidase (HRP) conjugated estradiol. The competitive inhibition reaction is launched between with HRP labeled estradiol and unlabeled estradiol with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of estradiol in the sample. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13710209	KTE0003	48 T	$189		Add to Cart
BHE13710209	KTE0003	96 T	$289		Add to Cart

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