EasyStep Human VEGF165(Vascular Endothelial Growth Factor 165) ELISA Kit

Mfr.No.

ELK027ES

Sensitivity

15 pg/ml

Detection Range

31.25 pg/mL - 2000 pg/mL

Reactivity

Human

Assay Duration

1.5h

Assay Type

Sandwich

Alternative Names

VEGF165

Recovery

Matrices listed below were spiked with certain level of recombinant VEGF165 and the recovery rates were calculated by comparing the measured value to the expected amount of VEGF165 in samples.

Matrix	Recovery range	Average
serum(n=5)	95-108%	101%
EDTA plasma(n=5)	83-97%	90%
Heparin plasma(n=5)	80-97%	88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF165 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-97%	87-96%	85-96%	85-95%
EDTA plasma(n=5)	95-106%	87-96%	82-90%	92-101%
Heparin plasma(n=5)	85-92%	95-103%	97-105%	93-102%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
2000.00	2.126	2.033
1000.00	1.731	1.638
500.00	1.114	1.021
250.00	0.855	0.762
125.00	0.532	0.439
62.50	0.335	0.242
31.25	0.226	0.133
0.00	0.093	0.000

Limit Of Detection

2000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGF165, and the Human VEGF165 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGF165. After TMB substrate solution is added, only those wells that contain Human VEGF165 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGF165 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Cytokine;Tumor immunity;

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15208350	ELK027ES	48T	$320		Add to Cart
BHE15208350	ELK027ES	96T	$370		Add to Cart

EasyStep Human VEGF165(Vascular Endothelial Growth Factor 165) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of EasyStep Human VEGF165(Vascular Endothelial Growth Factor 165) ELISA Kit

Rate it now..!!