E2(Estradiol) ELISA Kit

Mfr.No.

EU0390

Alternative Names

E2 ELISA Kit, Estradiol ELISA Kit

Reactivity

Universal

Detection Method

Coated with Antigen

Detection Range

12.5-800pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Competitive

Recovery

Matrices listed below were spiked with certain level of E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	87-103	95
EDTA plasma(n=5)	91-105	98
heparin plasma(n=5)	88-104	99

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-101%	97-103%	88-100%
EDTA plasma(n=5)	85-101%	84-101%	85-100%
heparin plasma(n=5)	81-96%	88-98%	82-95%

Sensitivity

< 7.5pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

E2(Estradiol)

Notes

For Research Use Only

Antigen	E2

Applications	ELISA
Applications Notes	E2 ELISA Kit allows for the in vitro quantitative determination of E2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

Reference	Journal: Iraqi Journal of Veterinary Sciences Title: Reproductive hormones profile of Iraqi Awassi ewes immunized against synthetic inhibin-α subunit or steroid-free bovine follicular fluid

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SKU	Supplier No.	Size	Your price	Quantity
BHE10804891	EU0390	96T	$350		Add to Cart

E2(Estradiol) ELISA Kit

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