General E1S(Estrone Sulfate) ELISA Kit

Mfr.No.

ELK7928

Alternative Names

E1S

Reactivity

General

Detection Range

1.57-100 ng/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Competitive Inhibition

Assay Duration

Recovery

Matrices listed below were spiked with certain level of recombinant E1S and the recovery rates were calculated by comparing the measured value to the expected amount of E1S in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	97-105%	101%
Heparin plasma(n=5)	83-99%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E1S and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	87-98%	85-92%	87-101%	89-99%
EDTA plasma(n=5)	88-104%	84-98%	95-105%	88-102%
Heparin plasma(n=5)	89-99%	79-96%	89-101%	85-97%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
100.00	0.232
50.00	0.397
25.00	0.689
12.50	0.856
6.25	1.139
3.13	1.596
1.57	1.799
0.00	2.325

Sensitivity

0.51 ng/mL

Limit Of Detection

100 ng/mL

Antigen	E1S
Usage Notes	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with E1S protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E1S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E1S in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Reproductive science;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200418	ELK7928	48T	$265		Add to Cart
BHE15200418	ELK7928	96T	$376		Add to Cart

E1S(Estrone Sulfate) ELISA Kit

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