Dog sRANKL(SolubleReceptor Activator of Nuclear factor-kB Ligand) ELISA Kit

Mfr.No.

ELK9165

Sensitivity

5.7 pg/mL

Detection Range

15.63-1000 pg/mL

Reactivity

Dog

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine; RANkL; Receptor Activator Of Nuclear Factor Kappa B Ligand

Recovery

Matrices listed below were spiked with certain level of recombinant sRANKL and the recovery rates were calculated by comparing the measured value to the expected amount of sRANKL in samples.

Matrix	Recovery range	Average
serum(n=5)	80-97%	88%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	87-95%	91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sRANKL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	98-105%	92-101%	85-105%	92-104%
EDTA plasma(n=5)	87-93%	95-106%	88-103%	81-98%
Heparin plasma(n=5)	82-96%	87-105%	90-99%	83-96%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.035	1.949
500.00	1.527	1.441
250.00	1.115	1.029
125.00	0.892	0.806
62.50	0.516	0.430
31.25	0.315	0.229
15.63	0.258	0.172
0.00	0.086	0.000

Limit Of Detection

1000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog sRANKL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog sRANKL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog sRANKL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog sRANKL in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Signal transduction;Metabolic pathway;Apoptosis;Tumor immunity;Bone metabolism;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15207366	ELK9165	48T	$320		Add to Cart
BHE15207366	ELK9165	96T	$458		Add to Cart

Dog sRANKL(SolubleReceptor Activator of Nuclear factor-kB Ligand) ELISA Kit

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