Dog sCD14(SolubleCluster of Differentiation 14) ELISA Kit

Mfr.No.

ELK9676

Alternative Names

CD14 Antigen, Monocyte Differentiation Antigen CD14, CD14 Antigen; Monocyte Differentiation Antigen CD14

Reactivity

Dog

Detection Range

1.56-100 ng/mL, 1.56-100 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant sCD14 and the recovery rates were calculated by comparing the measured value to the expected amount of sCD14 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-95%	91%
EDTA plasma(n=5)	77-95%	86%
Heparin plasma(n=5)	87-99%	92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sCD14 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	92-101%	80-94%	86-101%	89-98%
EDTA plasma(n=5)	81-94%	96-105%	88-97%	86-102%
Heparin plasma(n=5)	91-103%	89-97%	85-102%	87-96%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
100.00	2.054	1.969
50.00	1.587	1.502
25.00	1.088	1.003
12.50	0.757	0.672
6.25	0.579	0.494
3.13	0.325	0.240
1.57	0.258	0.173
0.00	0.085	0.000

Sensitivity

0.94 ng/mL

Limit Of Detection

100 ng/mL

Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog sCD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog sCD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog sCD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog sCD14 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Immunology;Stem Cells;Kits/ Lysates/ Other
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE152011411	ELK9676	48T	$320		Add to Cart
BHE152011411	ELK9676	96T	$458		Add to Cart

Dog sCD14(SolubleCluster of Differentiation 14) ELISA Kit

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