Dog N-terminal Pro atrial natriuretic peptide (NT-ProANP)ELISA kit

Mfr.No.

CSB-EQ027254DO

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Canis familiaris, Dog

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

N-terminal Pro atrial natriuretic peptide (NT-ProANP)

Recovery

The recovery of guinea pig cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-101
EDTA plasma (n=4)	88	84-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	103
	Range %	99-109
1:4	Average %	90
	Range %	85-94
1:8	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	NT-ProANP

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Serum Levels of CardiacMarkers NT-proANP and NT-proBNP in Brachycephalic bitches at Different Gestational Stages Feliciano M.et al,Reprod Domest Anim.,2016 Mar 16.

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