Dog matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

Dog matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

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Mfr.No.

CSB-E13887c

Sensitivity

3.12 ng/mL

Detection Range

12.5 ng/mL-800 ng/mL

Reactivity

Canis familiaris, Dog

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), CLG4B, GELB, MANDP2, MMP-9, macrophage gelatinase, matrix metalloproteinase 9, matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase), type V collagenase

Recovery

The recovery of rat ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-99
EDTA plasma (n=4)	88	80-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-95
1:2	Average %	101
	Range %	96-105
1:4	Average %	93
	Range %	88-97
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.312	2.456	2.384	2.237
10	1.586	1.712	1.649	1.502
5	1.009	1.054	1.032	0.885
2.5	0.641	0.632	0.637	0.490
1.25	0.412	0.435	0.424	0.277
0.625	0.287	0.274	0.281	0.134
0.312	0.211	0.218	0.215	0.068
0	0.146	0.147	0.147

Species

Canis lupus familiaris

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O18733

Applications	ELISA
Research Area	MMP9

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10500613	CSB-E13887c	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.