Dog Interleukin 2,IL-2 ELISA Kit

Mfr.No.

CSB-E11258c

Alternative Names

interleukin 2, IL-2, TCGF, lymphokine, T cell growth factor, aldesleukin, interleukin-2, involved in regulation of T-cell clonal expansion

Reactivity

Canis familiaris, Dog

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of sheep TGFB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-101
EDTA plasma (n=4)	97	87-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep TGFB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-102
1:2	Average %	90
	Range %	82-99
1:4	Average %	95
	Range %	91-99
1:8	Average %	97
	Range %	93-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q29416
Antigen	Interleukin 2

Applications

ELISA

Research Area

IL2

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500600	CSB-E11258c	96T	$595	Add to Cart
BHE10500600	CSB-E11258c	5x96T	$2856	Add to Cart
BHE10500600	CSB-E11258c	10x96T	$5652.50	Add to Cart