Dog intercellular adhesion molecule 1,ICAM-1/CD54 ELISA Kit

Dog intercellular adhesion molecule 1,ICAM-1/CD54 ELISA Kit

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Mfr.No.

CSB-E08898c

Sensitivity

6.25 ng/mL

Detection Range

25 ng/mL-1600 ng/mL

Reactivity

Canis familiaris, Dog

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

intercellular adhesion molecule 1, BB2, CD54, P3.58, cell surface glycoprotein P3.58, human rhinovirus receptor, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor

Recovery

The recovery of rat cTn-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-92
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-98
1:2	Average %	98
	Range %	91-104
1:4	Average %	99
	Range %	94-105
1:8	Average %	87
	Range %	82-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.321	2.220	2.271	2.118
1000	1.340	1.441	1.391	1.238
500	0.814	0.835	0.825	0.672
250	0.529	0.540	0.535	0.382
125	0.345	0.334	0.340	0.187
62.5	0.261	0.250	0.256	0.103
31.25	0.197	0.193	0.195	0.042
0	0.153	0.152	0.153	?

Species

Canis lupus familiaris

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P33729

Applications	ELISA
Research Area	ICAM1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500586	CSB-E08898c	96T	$595	Add to Cart
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BHE10500586	CSB-E08898c	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.