Dog GMCSF(Colony Stimulating Factor 2, Granulocyte Macrophage) ELISA Kit

Mfr.No.

ELK5675

Alternative Names

CSF2; GM-CSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor

Reactivity

Dog

Detection Range

7.82-500 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant GMCSF and the recovery rates were calculated by comparing the measured value to the expected amount of GMCSF in samples.

Matrix	Recovery range	Average
serum(n=5)	86-99%	92%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	83-98%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GMCSF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	91-97%	86-92%	79-93%	89-106%
EDTA plasma(n=5)	85-100%	80-97%	81-92%	87-103%
Heparin plasma(n=5)	82-93%	95-104%	82-96%	85-98%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
500.00	2.231	2.128
250.00	1.599	1.496
125.00	1.194	1.091
62.50	0.928	0.825
31.25	0.525	0.422
15.63	0.388	0.285
7.82	0.252	0.149
0.00	0.103	0.000

Sensitivity

3.1 pg/mL

Limit Of Detection

500 pg/mL

UniProt ID	P48749
Antigen	GMCSF
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog GMCSF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog GMCSF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog GMCSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog GMCSF in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Cytokine;Hematology;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15207462	ELK5675	48T	$412		Add to Cart
BHE15207462	ELK5675	96T	$588		Add to Cart

Dog GMCSF(Colony Stimulating Factor 2, Granulocyte Macrophage) ELISA Kit

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