Dog Endothelin 1,ET-1 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07002c

Alternative Names

endothelin 1, ET1, HDLCQ7, preproendothelin 1

Reactivity

Canis familiaris, Dog

Detection Range

3.12 pg/mL-200 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	94
	Range %	90-98
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Sensitivity

0.78 pg/mL

Limit Of Detection

450 nm

UniProt ID	P13206
Antigen	Endothelin 1

Applications

ELISA

Research Area

EDN1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10500563	CSB-E07002c	96T	$695	Add to Cart
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